Postdoctoral researcher (initial contract 2 years renewable up to 3 years) in charge of developing and conducting experimental protocols, as part of research projects on the links between mechanical forces and cellular metabolism. The spatio-temporal control of cellular behavior requires the transmission of information from the complex structure of tissues to the cells that constitute them. Mechanotransduction allows this transmission by detecting the mechanical environment and adapting cellular behavior. However, this process requires energy. Since 2016 and our pioneering study demonstrating a link between cellular mechanics and cellular metabolism (Bertero et al, J Clin Invest., 2016), our laboratory has been interested in deciphering how the mechanical properties of tissues shape - and are shaped by - cellular metabolism (Torrino and Bertero. Trends Cell. Biol. 2022). To this end, we exploit a combination of biophysical and biochemical approaches (AFM, STED, fluxomics, proteomics…), coupled with 3D cell culture models (organoids), genetically modified rodent models (breast cancer and pulmonary hypertension models) and patient biopsies. Thanks to our translational discovery platform, we were the first to identify the mechano-induced metabolic cooperation of cells in the pulmonary vasculature (Bertero et al., Cell Rep. 2015, Bertero et al., J Clin Invest 2016; Rachedi et al Cell Metab. 2024) and the tumor niche (Bertero et al., Cell Metab., 2019; Torrino et al., Cell Metab, 2021)

- Study of cellular metabolism
- Study of the mechanical properties of the cell/tissue
- Setting up iPSC culture.
- Biochemical approach (protein purification, enzymatic test, etc.)
- 2D/3D line/primary cell culture
- Analysis of cellular behavior (morphology, proliferation, migration, etc.)
- Confocal/super-resolution microscopy analysis
- Formatting of results and statistical analysis.
- Monitoring of the literature and improvement/adaptation of techniques and analyses according to research projects.

The position is to be filled within the Institute of Molecular and Cellular Pharmacology in Valbonne – Sophia Antipolis (Alpes-Maritimes). The IPMC is a joint research unit (UMR 7275) formed between the CNRS, Inserm, and the Université Côte d'Azur (250 people, 8000 m2 of buildings). Its 22 research teams benefit from a high-level technological environment in integrative biology, molecular and cellular biology, imaging, cytometry, analysis of biomolecules, functional genomics as well as common technical platforms for proteomics and metabolomics. The postdoc will work in the team "Cellular mechanics and metabolism: From tissue to molecule" under the direction of Thomas BERTERO.

The position is located in a sector covered by the protection of scientific and technical potential (PPST), and therefore requires, in accordance with the regulations, that your arrival be authorized by the competent authority of the MESR.